Generator

Part:BBa_I761005:Design

Designed by: 2007 NYMU_Taiwan iGEM Team   Group: iGEM07_Taipei   (2007-10-19)


Glucose controlled insulin generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The A and B chain of human insulin are encoded separately because E. coli lack human post-translational modification mechanism. To ensure proper disulfide bond formation, this generator must be transformed into periplasmic oxidase mutant strain like DR473.


Source

  • BBa_B0034
  • BBa_R0082
  • BBa_I761002
  • BBa_I761003

References